Microbead emulsion pcr8/13/2023 Mahalanabis M, Do J, Almuayad H et al (2010) An integrated disposable device for DNA extraction and helicase dependent amplification. doi: 10.1016/j.ab.2006.05.005Ĭhen Y, Seo TS (2011) PCR-free digital minisatellite tandem repeat genotyping. doi: 10.1021/ac3016533Īrcher MJ, Lin B, Wang Z, Stenger DA (2006) Magnetic bead-based solid phase for selective extraction of genomic DNA. Hwang K-Y, Kwon SH, Jung S-O et al (2012) Solid phase DNA extraction with a flexible bead-packed microfluidic device to detect methicillin-resistant Staphylococcus aureus in nasal swabs. Hwang K-Y, Kwon SH, Jung S-O et al (2011) Miniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria. doi: 10.1016/j.bios.2008.09.015Ĭhou J, Wong J, Christodoulides N et al (2012) Porous bead-based diagnostic platforms: bridging the gaps in healthcare. Contains 211 references.įilipponi L, Sawant PD, Fulga F, Nicolau DV (2009) Microbeads on microposts: an inverted architecture for bead microarrays. We finally discuss the relevance and trends of the methods in terms of high-level multiplexed analysis and their potential in diagnosis and personalized medicine. We discuss selected trends such as microbead-coupled amplification, heterogeneous and homogenous DNA hybridization assays, real-time assays, melting curve analysis, and digital microbead assays. Specific sections cover chemical properties of microbeads, the coupling of targets onto solid surfaces, microbead probe systems (mainly oligonucleotide probes), microbead detection schemes (with subsections on suspension arrays, microfluidic devices, and immobilized microbeads), quantification of nucleic acids, PCR in solution and the detection of amplicons, and methods for solid-phase amplification. This review provides an overview on microbead-based platforms for biosensing with a main focus on nucleic acid detection (including amplification strategies and on selected probe systems using fluorescent labeling). The potential of microbead-based assays therefore is apparent, and commercial applications can be found in the detection and discrimination of single nucleotide polymorphism, of pathogens, and in trapping assays. Its advances include low costs, low workload, high speed and high-throughput automation. Microbead assays have been improved in the past decades and are now important tools in routine and point-of-care diagnostics as well as in life science. Nucleic acid-based methods include hybridization assays, solid-phase PCR, sequencing, and trapping assays. They also form the basis of various techniques for detection and quantification of nucleic acids and proteins. Microbead-based technologies represent elegant and versatile approaches for highly parallelized quantitative multiparameter assays.
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